ETHYL TEST
SALIVA ALCOHOL TEST

The rapid Ethyl Test is an enzymatic test for the quick and reliable detection of the presence of alcohol (alc) (ethyl alcohol/ethanol) in saliva and other liquid samples. By analyzing the saliva sample, it is possible to determine the blood alcohol concentration.
This enzymatic analysis provides a qualitative result, meaning the test indicates whether alcohol is present in the samples.
The analysis detects an ethanol concentration of 0.1% or 10 mg/dL, which is indicated by a light grey-green discoloration of the reactive pad or test pad. The green color becomes more intense as the alcohol concentration in the sample increases. Therefore, in the presence of alcohol in the sample, the Ethyl Test shows a color change ranging from light grey-green for an alcohol concentration of 0.1%, progressing to a slightly more intense green with an average concentration, up to a dark grey-green color if the alcohol concentration approaches 1.50%.
We recommend using this visual test only as a qualitative test, as the human eye's ability to discriminate can lead to errors in quantitative results.
Measures 5 levels of alcohol in the body: 0.00 g/l - 0.20 g/l - 0.50 g/l - 0.80 g/l - 1.50 g/l.
The Ethyl Test consists of a plastic stick with a reactive pad at its tip. When in contact with alcohol solutions, the pad rapidly changes color from green to blue, depending on the amount of alcohol in the liquid. The reactive pad reacts based on a solid-phase chemical synthesis.

How to use
1. Do not put anything in your mouth for fifteen (15) minutes before starting the test. This warning includes non-alcoholic beverages, tobacco products, coffee, breath mints, food, etc.
2. Bring the sealed pouch to room temperature (15-30°C) to avoid moisture condensation on the reactive pad.
3. If analyzing liquid samples other than saliva, ensure they are at room temperature before proceeding with the test.
4. Open the package and remove the stick. Observe the reactive pad at the end of the stick. The pad should be light cream in color. A stick with a dark brown or stained reactive pad should be discarded.
5. Saturate the reactive pad with the liquid or with saliva collected from the mouth or a spittoon. Start the timer immediately.
6. After two (2) minutes, observe the color change (if any) on the reactive pad. If the pad turns green or dark grey-green, it means the test has detected the presence of alcohol, and the result is positive. Results obtained after more than 3 minutes may be inaccurate.
7. Estimate the approximate blood alcohol concentration by comparing the color of the reactive pad with the color scale on the test package. We recommend using the visual test only as a qualitative test.

Interpretation of results
After 2 minutes, read the color of the reactive pad. Do not compare the stick's result with other color scales. Use only the color scale provided on the package. The reaction occurs more slowly in saliva than in other aqueous solutions.

Limitations
If, after ingesting food, beverages, or anything else, you do not wait at least 15 minutes before performing the test, the results could be inaccurate due to possible contamination of the saliva with interfering substances.
The Ethyl Test has been designed and calibrated to be interpreted 2 minutes after saturating the reactive pad. If you wait more than 2 minutes, the results shown may be inaccurate or false positive results may occur.
The Ethyl Test can be used to detect the presence of alcohol in fluids other than saliva. However, if used in this way, the color scale on the package cannot be used as a reference.
If alcohol is present in the fluid, the pad's color will range from light grey-green to black or cocoa, as the alcohol concentration increases. If the pad is immersed in pure alcohol, the pad will change color slightly or not at all, due to the absence of water which is necessary for the coloring reaction to occur.
When analyzing beverages, the result can only be considered positive if the pad turns very dark brown.
The Ethyl Test is very sensitive to alcohol. Alcohol vapors often present in the air are sometimes detected by the Ethyl Test.
Alcohol vapors are often present in many public places and inside homes. Alcohol is found in many household cleaning products such as disinfectants, deodorants, and glass cleaners. If the presence of alcohol vapors is suspected, the test should be performed in an area where these vapors are not present (outdoors, for example).
Note: the person reading the test results cannot be colorblind.

Controls
The integrity of the Ethyl Test can be qualitatively checked using a solution prepared by adding 4 drops of 40% alcohol by volume distilled liquor to a glass of water.
With this solution, the coloration should correspond to the window equal to or greater than 0.04% (darker).
The color reaction with alcohol in saliva occurs more slowly and with less intense coloration compared to alcohol in aqueous solutions.

Features
Specificity: the ethyl test will react in the presence of methyl, ethyl, and allyl alcohol. The Ethyl Test will not produce any reaction with alcohols having 5 or more carbon atoms, nor with glycine, glycerol, or serine.
This property derives from the specificity of the alcohol oxidase enzyme extracted from yeast.

Interactions
The following substances can interfere with the Ethyl Test stick if liquid samples other than saliva are used:
Agents that intensify coloration:
- Peroxide.
- Strong oxidizers.
- (common in detergents, pickling agents, and bleaches).
Agents that inhibit coloration:
Reducing agents:
- Ascorbic acid.
- Tannic acid.
- Pyrogallol.
- Mercaptans and tosylates.
- Oxalic acid.
- Uric acid.
- Bilirubin.
- L-dopamine.
- L-methyldopamine.
- Metamizole.
The aforementioned substances are usually not present in saliva in quantities sufficient to interfere with the test. However, it is necessary that they are not introduced into the mouth in the 15 minutes preceding the test.

Warnings
- Single use.
- Product not intended for medical or diagnostic purposes.
- Do not use beyond the expiration date.
- Allow the reactive pad to absorb enough liquid to fully saturate it.
- Do not eat, drink, or smoke while handling samples or the kit.
- Do not use the test if the pouch is damaged.
- Bring reagents to room temperature (15-30°C) before use.
- Do not touch the reactive pad on the stick to avoid contaminating it.
- Avoid cross-contamination between samples by using a new collection container for each sample.
- Not for medical or diagnostic use.
- Handle all samples as if they contain infectious agents. During the test, observe precautions against microbiological risks and follow standard procedures for proper disposal of samples.
- Leave the stick in the sealed pouch until use.
- Observe the test result after 2-3 minutes.
- Humidity and high temperatures can affect test results.
- Do not perform the test in an alcohol-containing atmosphere.
- Pure alcohol can give false results.
- Pay attention to possible cross-reactivities.
- We recommend using the visual test only as a qualitative test.
- The person reading the results cannot be colorblind.

Storage
The test kit should be stored in the refrigerator or at room temperature (2-30°C) in the sealed pouch, according to the product's shelf life.
Bring the stick to room temperature to avoid moisture condensation on the reactive pad.

Composition/Ingredient Information
Complete kit composition:
- Test strip: contains a filter paper pad with alcohol oxidase, peroxidase, 3,3,5,5-tetramethylbenzidine (TMB), and TRIS-HCl buffer in a dry state.
- The test strip consists of: PE catalyst, filter paper reagent pad.
- Other components: aluminum pouch, hygroscopic silica gel.
Harmful components:
none.

Format
Pack of 1 piece.

Code. CALC1S